How to store PBS
- Store sealed sterile PBS at room temperature or as stated on the label.
- Keep containers tightly closed, and open them only with aseptic technique.
- Many labs refrigerate opened PBS to slow microbial growth. Let it warm before use with live cells.
- Concentrated (10X) PBS can form crystals when cold. Warm and mix it until fully dissolved before diluting.
How long does PBS last?
Use the expiry date on the label and certificate of analysis for your lot. After opening, how long it stays usable depends on how it’s handled: every entry into the container risks contamination.
Signs PBS should be discarded
- Cloudiness, floating particles or film
- Crystals that don’t redissolve after warming
- Any color change
- Past the labeled expiry
Why small sterile vials help
Small vials mean less repeated opening of one large bottle and less waste when a container is compromised. Our 10 mL sterile PBS vials come with lot tracking, and the current lot is documented at pH 7.37 and ≤0.05 EU/mL endotoxin.
More PBS guides
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